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Article Navigation >  PETROLEUM GEOLOGY & EXPERIMENT  > 2014  >  36(5): 605-611
Tang Yuping, Gao Junyang, Zhao Kebin, Ren Chun, Xu Kewei. Molecular biological analysis of methanotrophic bacterial community above typical oil and gas reservoirs[J]. PETROLEUM GEOLOGY & EXPERIMENT, 2014, 36(5): 605-611. doi: 10.11781/sysydz201405605
Citation: Tang Yuping, Gao Junyang, Zhao Kebin, Ren Chun, Xu Kewei. Molecular biological analysis of methanotrophic bacterial community above typical oil and gas reservoirs[J]. PETROLEUM GEOLOGY & EXPERIMENT, 2014, 36(5): 605-611. doi: 10.11781/sysydz201405605
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Molecular biological analysis of methanotrophic bacterial community above typical oil and gas reservoirs

doi: 10.11781/sysydz201405605

  • Wuxi Research Institute of Petroleum Geology, SINOPEC, Wuxi, Jiangsu 214151, China

  • Received Date: 2013-05-14
  • Rev Recd Date: 2014-07-31
  • Publish Date: 2014-09-28
    Abstract
  • In this study, the community structure of aerobic methanotrophic communities were investigated at light hydrocarbon microseeps above a known oil band gas field in the Shengli Oilfield. Clone library analysis of the pmoA gene revealed major differences in the methanotrophic community composition between oil and gas fields and the surrounding soils. The relative abundance of methanotrophic Methylocystis and Methylosinus (type Ⅱ) in surrounding soils is obviously lower in oil and gas field soils than surrounding soils, ut the relative abundance of methanotrophic Methy-lococcus and Methylocaldum (type I) presents a reverse tread. Terminal restriction fragment length polymorphism (T-RFLP) analysis confirmed that one distinct Methylococcus and Methylobacter-related group may dominate the community at hydrocarbon microseeps. The pmoA gene copy numbers determined by real time PCR also indicated that methane oxidizing microbial community anomalies above typical oil and gas reservoirs exist objectively.

     

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    • References(33)
  • [1]
    Brisbane P G,Ladd J N.The role of microorganisms in petroleum exploration[J].Annual Review of Microbiology,1965,19:351-364.
    [2]
    Tedesco S A.Surface geochemistry in petroleum exploration[M].New York:Chapman & Hall,1995.
    [3]
    袁志华,许晨.松辽盆地西部杜75块油气微生物勘探研究[J].特种油气藏,2012,19(1):47-50. Yuan Zhihua,Xu Chen.Research on microbial prospecting for oil and gas in Block Du75 of western Songliao Basin[J].Special Oil & Gas Reservoirs,2012,19(1):47-50.
    [4]
    O'Malley M A.The nineteenth century roots of ‘everything is everywhere’[J].Nature Reviews Microbiology,2007,5(8):647-651.
    [5]
    Ehrlich H L.Geomicrobiology[M].New York:Marcel Dekker,2002.
    [6]
    Pérez-de-Mora A,Schulz S,Schloter M.MPN-and real-time-based PCR methods for the quantification of alkane monooxygenase homologous genes (alkB) in environmental samples[M]//Cummings S.Methods in molecular biology.New York:Humana Press,2010:59-68.
    [7]
    Redmond M C,Valentine D L,Sessions A L.Identification of novel methane-, ethane-, and propane-oxidizing bacteria at marine hydrocarbon seeps by stable isotope probing[J].Applied and Environmental Microbiology,2010,76(19):6412-6422.
    [8]
    Rojo F.Degradation of alkanes by bacteria[J].Environmental Microbiology,2009,11(10):2477-2490.
    [9]
    Zhang F,She Y H,Zheng Y.Molecular biologic techniques applied to the microbial prospecting of oil and gas in the Ban 876 gas and oil field in China[J].Applied Microbiology and Biotechnology,2010,86(4):1183-1194.
    [10]
    易绍金,熊汉辉,陈斌强.细菌瓶法用于油气微生物勘探中气态烃氧化菌菌数测定[J].油田化学,2006,23(1):92-95. Yi Shaojin,Xiong Hanhui,Chen Binqiang.Determination of bacteria cell population for gaseous hydrocarbon-oxidating bacteria by using culture flask test in Microbiological Prospecting of Oil and Gas (MPOG)[J].Oilfield Chemistry,2006,23(1):92-95.
    [11]
    Han B,Chen Y,Abell G,et al.Diversity and activity of methanotrophs in alkaline soil from a Chinese coal mine[J].FEMS Microbiology Ecology,2009,70(2):40-51.
    [12]
    Han B,Su T,Li X,et al.Research progresses of methanotrophs and methane monooxygenases[J].Chinese Journal of Biotechnology,2008,24(9):1511-1519.
    [13]
    田建华,苏朝光,张营革.陈家庄凸起西段陈22块东营组地层超覆油藏地震描述技术[J].油气地质与采收率,2008,15(3):50-53. Tian Jianhua,Su Chaoguang,Zhang Yingge,et al.Seismic description techniques for stratigraphic overlap oil reservoirs of Dongying Formation,Chen22 Block,western Chenjiazhuang Uplift[J].Petroleum Geology and Recovery Efficiency,2008,15(3):50-53.
    [14]
    Limpiyakorn T,Kurisu F,Yagi O.Development and application of real-time PCR for quantification of specific ammonia-oxidizing bacteria in activated sludge of sewage treatment systems[J].Applied Microbiology and Biotechnology,2006,72(5):1004-1013.
    [15]
    López-Gutiérrez J C,Henry S,Hallet S,et al.Quantification of a novel group of nitrate-reducing bacteria in the environment by real-time PCR[J].Journal of Microbiological Methods,2004,57(3):399-407.
    [16]
    Zhang T,Fang H.Applications of real-time polymerase chain reaction for quantification of microorganisms in environmental samples[J].Applied Microbiology and Biotechnology,2007,70(3):281-289.
    [17]
    Kemp P,Aller J.Estimating prokaryotic diversity:when are 16S rDNA libraries large enough?[J].Limnology and Oceanography:Methods,2004,2:114-125.
    [18]
    Peng J,Lü Z,Rui J,et al.Dynamics of the methanogenic archaeal community during plant residue decomposition in an anoxic rice field soil[J].Applied and Environmental Microbiology,2008,74(9):2894-2901.
    [19]
    Xu K W,Liu H,Li X F,et al.Typical methanogenic inhibitors can considerably alter bacterial populations and affect the interaction between fatty acid degraders and homoacetogens[J].Applied Microbiology and Biotechnology,2010,87:2267-2279.
    [20]
    Krista K M,Bonaunet K,Berland H,et al.Characterization of culture independent and dependent microbial communities in a high-temperature off shore chalk petroleum reservoir[J].Antonie Van Leeuwenhoek,2009,96(4):423-439.
    [21]
    Lysnes K,Bdtker G,Torsvik T,et al.Microbial response to reinjection of produced water in an oil reservoir[J].Applied and Environmental Microbiology,2009,83(6):1143-1157.
    [22]
    Andrews J H,Harris R F.R-selection and K-selection and microbial ecology[J].Advances in Microbial Ecology,1986,9:99-147.
    [23]
    Krause S,Lüke C,Frenzel P.Succession of methanotrophs in oxygen-methane counter-gradients of flooded rice paddies[J].The ISME Journal,2010,4(12):1603-1607.
    [24]
    Striegl R G,Mcconnaughey T A,Thorstenson D C,et al.Consumption of atmospheric methane by desert soils[J].Nature,1992,357(6374):145-147.
    [25]
    Valentine D L.Emerging topics in marine methane biogeoche-mistry[J].Annual Review of Marine Science,2011,3:147-171.
    [26]
    Boeckx P,Cleemput O V.Methane oxidation in a neutral landfill cover soil:Influence of moisture content,temperature,and nitrogen-turnover[J].Journal of Environmental Quality,1996,25(1):178-183.
    [27]
    Bowden R D,Newkirk K M,Rukko G M.Carbon dioxide and methane fluxes by a forest soil under laboratory-controlled moisture and temperature conditions[J].Soil Biology and Biochemistry,1998,30(12):1591-1597.
    [28]
    Hanson R S,Hanson T E.Methanotrophic bacteria[J].Microbiological Reviews,1996,60(2):439-471.
    [29]
    Hunger S,Schmidt O,Hilgarth M,et al.Competing formate- and carbon dioxide-utilizing prokaryotes in an anoxic methane-emitting fen soil[J].Applied and Environmental Microbiology,2011,77(11):3773-3785.
    [30]
    Deutzmann J S,Worner S,Schink B.Activity and diversity of methanotrophic bacteria at methane seeps in eastern lake constance sediments[J].Applied and Environmental Microbiology,2011,77(8):2573-2581.
    [31]
    ter Braak C J F,Ŝmilauer P.CANOCO reference manual and CanoDraw for Windows user's guide:Software for Canonical Community Ordination (version 4.5)[M].Microcomputer Power,NY,USA.2002.
    [32]
    Wendlandt K D,Stottmeister U,Helm J,et al.The potential of methane-oxidizing bacteria for applications in environmental biotechno-logy[J].Engineering in Life Sciences,2010,10(2):87-102.
    [33]
    Ward N,Larsen θ,Sakwa J,et al.Genomic insights into methanotrophy:The complete genome sequence of Methylococcus capsulatus (bath)[J].PLoS Biology,2004,2(10):1616-1628.
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