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典型油气藏上方甲烷氧化菌群的分子生物学解析

汤玉平 高俊阳 赵克斌 任春 许科伟

汤玉平, 高俊阳, 赵克斌, 任春, 许科伟. 典型油气藏上方甲烷氧化菌群的分子生物学解析[J]. 石油实验地质, 2014, 36(5): 605-611. doi: 10.11781/sysydz201405605
引用本文: 汤玉平, 高俊阳, 赵克斌, 任春, 许科伟. 典型油气藏上方甲烷氧化菌群的分子生物学解析[J]. 石油实验地质, 2014, 36(5): 605-611. doi: 10.11781/sysydz201405605
Tang Yuping, Gao Junyang, Zhao Kebin, Ren Chun, Xu Kewei. Molecular biological analysis of methanotrophic bacterial community above typical oil and gas reservoirs[J]. PETROLEUM GEOLOGY & EXPERIMENT, 2014, 36(5): 605-611. doi: 10.11781/sysydz201405605
Citation: Tang Yuping, Gao Junyang, Zhao Kebin, Ren Chun, Xu Kewei. Molecular biological analysis of methanotrophic bacterial community above typical oil and gas reservoirs[J]. PETROLEUM GEOLOGY & EXPERIMENT, 2014, 36(5): 605-611. doi: 10.11781/sysydz201405605

典型油气藏上方甲烷氧化菌群的分子生物学解析

doi: 10.11781/sysydz201405605
基金项目: 中国石化科技部项目(P11058)资助。
详细信息
    作者简介:

    汤玉平(1961-),男,博士、教授级高级工程师,从事油气地球化学勘探研究。E-mail:tangyuping.syky@sinopec.com。

  • 中图分类号: TE135

Molecular biological analysis of methanotrophic bacterial community above typical oil and gas reservoirs

  • 摘要: 以胜利油田典型油气藏为研究对象,原位提取土壤中的甲烷氧化微生物群落组成信息,明确了油气指示微生物类群。通过对油田、气田和背景区土壤的pmoA基因克隆文库分析发现,来自油气藏上方土壤与背景区土壤的甲烷氧化菌群结构的差异较大。背景区土壤的甲烷氧化Ⅱ型菌——甲基孢囊菌和甲基弯曲菌的相对丰度明显高于油气藏上方土壤,而甲烷氧化Ⅰ型菌——甲基球菌和甲基暖菌则显著减少,表明长期的油气微渗漏能使甲烷氧化微生物群落向Ⅰ型菌演替。T-RFLP多变量统计分析结果显示,经过地质历史时期的微量轻烃连续驯化,油气藏上方的甲烷氧化微生物群落发生了演替,甲基球菌和甲基杆菌可能是推动这种演替变化的关键种群。甲烷氧化pmoA基因的丰度在油气藏上方的异常可以用于预测下伏油气藏的存在。

     

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出版历程
  • 收稿日期:  2013-05-14
  • 修回日期:  2014-07-31
  • 刊出日期:  2014-09-28

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